Genotyping and primer details


Genotyping and primer details for 084C05 - At1g54020
Line ID 084C05
Locus At1g54020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20163503 of line 084C05 Chr1:20163503
   
Insertion allele amplicon 8474 - 08cy
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08cy: 5'-GTTTGCAATATTGAAGTACCCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20163199
   
wt amplicon (over InsPos) 16dw - 08cy
wt amplicon start Chr1:20163199
wt amplicon end Chr1:20164036
wt amplicon size 838 bp
Locus specific primer 08cy: 5'-GTTTGCAATATTGAAGTACCCGTT-3'
2nd locus specific primer 16dw: 5'-CATTTTTGCGAAACGTTTTATTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11