Genotyping and primer details


Genotyping and primer details for 085A06 - At5g66700
Line ID 085A06
Locus At5g66700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26635052 of line 085A06 Chr5:26635052
   
Insertion allele amplicon 8474 - JH02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JH02: 5'-GATGTTGAGGAAGAGGAAGTTGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26634609
   
Insertion allele amplicon 3144 - JH03
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer JH03: 5'-TCAAATGAGGAGGATATTTTCTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26635295
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11