Genotyping and primer details


Genotyping and primer details for 085G08 - At1g05160
Line ID 085G08
Locus At1g05160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1488435 of line 085G08 Chr1:1488435
   
Insertion allele amplicon 3144 - 01em
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 01em: 5'-GATCAAATTTTCTTGGATCTGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1488033
   
Insertion allele amplicon 3144 - 82wb
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 82wb: 5'-TGAGAGTGAGAATGTAATGGATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1489020
   
Insertion allele amplicon 3144 - FI37
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FI37: 5'-GATCAAATTTTCTTGGATCTGGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1488033
   
Insertion allele amplicon 3144 - mr22
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer mr22: 5'-TTTTCTTGGATCTGGAAAGACTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1488040
   
wt amplicon (over InsPos) 01em - MR22
wt amplicon start Chr1:1488033
wt amplicon end Chr1:1488775
wt amplicon size 743 bp
Locus specific primer MR22: 5'-GAAATCAAAGGAAGCAGAACATTT-3'
2nd locus specific primer 01em: 5'-GATCAAATTTTCTTGGATCTGGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11