Genotyping and primer details


Genotyping and primer details for 087C02 - At4g26650
Line ID 087C02
Locus At4g26650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13446105 of line 087C02 Chr4:13446105
   
Insertion allele amplicon 8474 - 10ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10ra: 5'-CTAGACGGTAAACCTCCAACAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13446522
   
wt amplicon (over InsPos) 11rt - 10ra
wt amplicon start Chr4:13445645
wt amplicon end Chr4:13446522
wt amplicon size 878 bp
Locus specific primer 10ra: 5'-CTAGACGGTAAACCTCCAACAAAG-3'
2nd locus specific primer 11rt: 5'-AATTGTTAGATGAAATGGGCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11