Genotyping and primer details


Genotyping and primer details for 088E02 - At1g48860
Line ID 088E02
Locus At1g48860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18069486 of line 088E02 Chr1:18069486
   
Insertion allele amplicon 8474 - 04wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04wu: 5'-GACACAAATACCGCAAAATTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18069114
   
wt amplicon (over InsPos) 34xo - 04wu
wt amplicon start Chr1:18069114
wt amplicon end Chr1:18070076
wt amplicon size 963 bp
Locus specific primer 04wu: 5'-GACACAAATACCGCAAAATTTCTT-3'
2nd locus specific primer 34xo: 5'-AGTAGTCAGTACTTGACCGCTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11