Genotyping and primer details


Genotyping and primer details for 088E06 - At1g48490
Line ID 088E06
Locus At1g48490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17923903 of line 088E06 Chr1:17923903
   
Insertion allele amplicon 8474 - 09sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09sm: 5'-GGGTTGCTTATTGATGTTTACGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17923578
   
wt amplicon (over InsPos) 11sz - 09sm
wt amplicon start Chr1:17923578
wt amplicon end Chr1:17924409
wt amplicon size 832 bp
Locus specific primer 09sm: 5'-GGGTTGCTTATTGATGTTTACGAT-3'
2nd locus specific primer 11sz: 5'-ACGCATTTCTTTCTCATCTTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11