Genotyping and primer details


Genotyping and primer details for 090C01 - At5g54200
Line ID 090C01
Locus At5g54200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21994296 of line 090C01 Chr5:21994296
   
Insertion allele amplicon 8474 - 14eq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14eq: 5'-GTTTCTCCATCTCGTCTTTTGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21994703
   
wt amplicon (over InsPos) 03fl - 14eq
wt amplicon start Chr5:21993858
wt amplicon end Chr5:21994703
wt amplicon size 846 bp
Locus specific primer 14eq: 5'-GTTTCTCCATCTCGTCTTTTGAAC-3'
2nd locus specific primer 03fl: 5'-GGTTATTATTGGCTGTGAAGGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11