Genotyping and primer details


Genotyping and primer details for 094C05 - At3g61390
Line ID 094C05
Locus At3g61390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22717587 of line 094C05 Chr3:22717587
   
Insertion allele amplicon 8474 - 40fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40fr: 5'-GACAAATAAAGTATTGTGGCGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22717945
   
wt amplicon (over InsPos) 22go - 40fr
wt amplicon start Chr3:22717179
wt amplicon end Chr3:22717945
wt amplicon size 767 bp
Locus specific primer 40fr: 5'-GACAAATAAAGTATTGTGGCGGTT-3'
2nd locus specific primer 22go: 5'-TATATGGTTTACGTGCAACGGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11