Genotyping and primer details


Genotyping and primer details for 095A05 - At1g78980
Line ID 095A05
Locus At1g78980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29710406 of line 095A05 Chr1:29710406
   
Insertion allele amplicon 8474 - DC04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DC04: 5'-CAACACTAACTTTGGAAACCCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29711313
   
wt amplicon (over InsPos) 17dw - DC04
wt amplicon start Chr1:29709888
wt amplicon end Chr1:29711313
wt amplicon size 1426 bp
Locus specific primer DC04: 5'-CAACACTAACTTTGGAAACCCATT-3'
2nd locus specific primer 17dw: 5'-GTATCCATCCTTCAAACTGGTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11