Genotyping and primer details


Genotyping and primer details for 098C02 - At3g03880
Line ID 098C02
Locus At3g03880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:998344 of line 098C02 Chr3:998344
   
Insertion allele amplicon 3144 - 52wr
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 52wr: 5'-TTATCCCCAAAAAGAGAGGAAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:997556
   
Insertion allele amplicon 8474 - BH73
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH73: 5'-TGGATCACTGGAATGAAATTGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:998891
   
wt amplicon (over InsPos) 08uo - BH73
wt amplicon start Chr3:997877
wt amplicon end Chr3:998891
wt amplicon size 1015 bp
Locus specific primer BH73: 5'-TGGATCACTGGAATGAAATTGATA-3'
2nd locus specific primer 08uo: 5'-CTGACGTCGTAGAATTCATCGTAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11