Genotyping and primer details


Genotyping and primer details for 099D10 - At1g60190
Line ID 099D10
Locus At1g60190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22199592 of line 099D10 Chr1:22199592
   
Insertion allele amplicon 8474 - BB72
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BB72: 5'-ATCACCGTAATCGCAACTTTTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22199956
   
wt amplicon (over InsPos) 19cu - BB72
wt amplicon start Chr1:22199310
wt amplicon end Chr1:22199956
wt amplicon size 647 bp
Locus specific primer BB72: 5'-ATCACCGTAATCGCAACTTTTAAC-3'
2nd locus specific primer 19cu: 5'-ATGATCGGAGCTCTATCACAAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11