Genotyping and primer details


Genotyping and primer details for 100H05 - At3g56350
Line ID 100H05
Locus At3g56350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20894548 of line 100H05 Chr3:20894548
   
Insertion allele amplicon 8474 - CD41
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD41: 5'-CATCTTCGGTACTGCCGTATTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20894081
   
wt amplicon (over InsPos) CB40 - CD41
wt amplicon start Chr3:20894081
wt amplicon end Chr3:20895026
wt amplicon size 946 bp
Locus specific primer CD41: 5'-CATCTTCGGTACTGCCGTATTTAT-3'
2nd locus specific primer CB40: 5'-TCATTTCGGATCACTAGAAGGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11