Genotyping and primer details


Genotyping and primer details for 101C11 - At5g38450
Line ID 101C11
Locus At5g38450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15396874 of line 101C11 Chr5:15396874
   
Insertion allele amplicon 8474 - MS67
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MS67: 5'-GAATTAAATGGAAACGTTTAGCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15397499
   
wt amplicon (over InsPos) 01lo - MS67
wt amplicon start Chr5:15396458
wt amplicon end Chr5:15397499
wt amplicon size 1042 bp
Locus specific primer MS67: 5'-GAATTAAATGGAAACGTTTAGCCA-3'
2nd locus specific primer 01lo: 5'-AGTTTATTGACGTGCCTTGAGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11