Genotyping and primer details


Genotyping and primer details for 101D04 - At5g63120
Line ID 101D04
Locus At5g63120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25321607 of line 101D04 Chr5:25321607
   
Insertion allele amplicon 8474 - UF25
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer UF25: 5'-GATCACGAATCTGAGGTCCTTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25320963
   
wt amplicon (over InsPos) 13fi - UF25
wt amplicon start Chr5:25320963
wt amplicon end Chr5:25322109
wt amplicon size 1147 bp
Locus specific primer UF25: 5'-GATCACGAATCTGAGGTCCTTTAG-3'
2nd locus specific primer 13fi: 5'-GAGCGAGAGATCAAAAACCATAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11