Genotyping and primer details


Genotyping and primer details for 101G09 - At3g13790
Line ID 101G09
Locus At3g13790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4534181 of line 101G09 Chr3:4534181
   
Insertion allele amplicon 3144 - NA89
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer NA89: 5'-TGTTGAAAATAAGTTTGGACGACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4534614
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11