Genotyping and primer details


Genotyping and primer details for 103E12 - At2g17000
Line ID 103E12
Locus At2g17000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:7389811 of line 103E12 Chr2:7389811
   
Insertion allele amplicon 8474 - 02ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02ht: 5'-AGAGAAGAGCACTTGCCTTAACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:7390187
   
wt amplicon (over InsPos) 03io - 02ht
wt amplicon start Chr2:7389405
wt amplicon end Chr2:7390187
wt amplicon size 783 bp
Locus specific primer 02ht: 5'-AGAGAAGAGCACTTGCCTTAACAC-3'
2nd locus specific primer 03io: 5'-CTCTCCCATGTTTTGATGGTTAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11