Genotyping and primer details


Genotyping and primer details for 103F10 - At3g21370
Line ID 103F10
Locus At3g21370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7525589 of line 103F10 Chr3:7525589
   
Insertion allele amplicon 3144 - VL06
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer VL06: 5'-TCCAAATGCCTAAAGAAAAAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7525365
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11