Genotyping and primer details


Genotyping and primer details for 104E05 - At1g09410
Line ID 104E05
Locus At1g09410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3036639 of line 104E05 Chr1:3036639
   
Insertion allele amplicon 3144 - FI53
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer FI53: 5'-TTCTCCGGTTCAATCTCTATGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3037043
   
Insertion allele amplicon 3144 - IS44
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IS44: 5'-AGTTATGCCAGTGAAGCCAGTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3036216
   
wt amplicon (over InsPos) FI53 - IS44
wt amplicon start Chr1:3036216
wt amplicon end Chr1:3037043
wt amplicon size 828 bp
Locus specific primer IS44: 5'-AGTTATGCCAGTGAAGCCAGTTAT-3'
2nd locus specific primer FI53: 5'-TTCTCCGGTTCAATCTCTATGAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11