Genotyping and primer details


Genotyping and primer details for 106F01 - At5g40390
Line ID 106F01
Locus At5g40390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16161939 of line 106F01 Chr5:16161939
   
Insertion allele amplicon 8474 - MS22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MS22: 5'-GAGCCATGTGACTAGAACCATGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16161357
   
wt amplicon (over InsPos) 13cu - MS22
wt amplicon start Chr5:16161357
wt amplicon end Chr5:16162468
wt amplicon size 1112 bp
Locus specific primer MS22: 5'-GAGCCATGTGACTAGAACCATGTA-3'
2nd locus specific primer 13cu: 5'-TCGACGAGACACTTAACACCTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11