Genotyping and primer details


Genotyping and primer details for 106F05 - At1g58030
Line ID 106F05
Locus At1g58030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21464484 of line 106F05 Chr1:21464484
   
Insertion allele amplicon 8474 - 15ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15ra: 5'-TCTGAGTTCCATAGATCAGGATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21464853
   
wt amplicon (over InsPos) 10rt - 15ra
wt amplicon start Chr1:21464163
wt amplicon end Chr1:21464853
wt amplicon size 691 bp
Locus specific primer 15ra: 5'-TCTGAGTTCCATAGATCAGGATGA-3'
2nd locus specific primer 10rt: 5'-AAAGAGATGCTGCTTTCTCAGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11