Genotyping and primer details


Genotyping and primer details for 106H05 - At1g03080
Line ID 106H05
Locus At1g03080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:733602 of line 106H05 Chr1:733602
   
Insertion allele amplicon 8474 - FI56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FI56: 5'-TGTAAAAAGCTCCTGGTTCAGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:733249
   
Insertion allele amplicon 3144 - MZ80
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer MZ80: 5'-AGACCCAGAAACTTATCTTCGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:734069
   
wt amplicon (over InsPos) FI56 - MZ80
wt amplicon start Chr1:733249
wt amplicon end Chr1:734069
wt amplicon size 821 bp
Locus specific primer MZ80: 5'-AGACCCAGAAACTTATCTTCGTGA-3'
2nd locus specific primer FI56: 5'-TGTAAAAAGCTCCTGGTTCAGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11