Genotyping and primer details


Genotyping and primer details for 107A06 - At4g11220
Line ID 107A06
Locus At4g11220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:6839022 of line 107A06 Chr4:6839022
   
Insertion allele amplicon 8474 - BH70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BH70: 5'-GTTTATCGTTTGTTCGGAAGAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6839383
   
Insertion allele amplicon 3144 - BH71
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer BH71: 5'-GAATAAGAGAGAAGGCAGAAGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:6838382
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11