Genotyping and primer details


Genotyping and primer details for 108C11 - At1g20823
Line ID 108C11
Locus At1g20823 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7239451 of line 108C11 Chr1:7239451
   
Insertion allele amplicon 8474 - KP35
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KP35: 5'-TGGCTCAATGCTAGTGACTAATTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7239967
   
Insertion allele amplicon 3144 - KP36
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KP36: 5'-CTCAAACTCAATCTCCTCAACCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7239085
   
wt amplicon (over InsPos) KP35 - KP36
wt amplicon start Chr1:7239085
wt amplicon end Chr1:7239967
wt amplicon size 883 bp
Locus specific primer KP36: 5'-CTCAAACTCAATCTCCTCAACCTC-3'
2nd locus specific primer KP35: 5'-TGGCTCAATGCTAGTGACTAATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11