Genotyping and primer details


Genotyping and primer details for 109A04 - At1g78680
Line ID 109A04
Locus At1g78680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29595450 of line 109A04 Chr1:29595450
   
Insertion allele amplicon 8474 - FB12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FB12: 5'-ATGTAAACCTCGTCGTAACCAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29596009
   
Insertion allele amplicon 3144 - HP70
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer HP70: 5'-GTTATGCAGAATCATCGGGTTAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29594955
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11