Genotyping and primer details


Genotyping and primer details for 109A09 - At3g24350
Line ID 109A09
Locus At3g24350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8838561 of line 109A09 Chr3:8838561
   
Insertion allele amplicon 8474 - TU07
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer TU07: 5'-TGTTAACTTTGTCGTTTTTGTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8838181
   
wt amplicon (over InsPos) 01kv - TU07
wt amplicon start Chr3:8838181
wt amplicon end Chr3:8839107
wt amplicon size 927 bp
Locus specific primer TU07: 5'-TGTTAACTTTGTCGTTTTTGTTGC-3'
2nd locus specific primer 01kv: 5'-CATGGTTGCTAGTTGTGTGAAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11