Genotyping and primer details


Genotyping and primer details for 110B11 - At4g25030
Line ID 110B11
Locus At4g25030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12866595 of line 110B11 Chr4:12866595
   
Insertion allele amplicon 8474 - CD58
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD58: 5'-CCGCTTAATTGTTGTTTCTCTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12865971
   
wt amplicon (over InsPos) 05ft - CD58
wt amplicon start Chr4:12865971
wt amplicon end Chr4:12866915
wt amplicon size 945 bp
Locus specific primer CD58: 5'-CCGCTTAATTGTTGTTTCTCTTTC-3'
2nd locus specific primer 05ft: 5'-GCAACCACGATATCATAAGCACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11