Genotyping and primer details


Genotyping and primer details for 110C12 - At3g03340
Line ID 110C12
Locus At3g03340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:788613 of line 110C12 Chr3:788613
   
Insertion allele amplicon 8474 - 09fb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09fb: 5'-CGATCATAATCTCTTCGATGTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:789513
   
wt amplicon (over InsPos) 11sq - 09fb
wt amplicon start Chr3:788242
wt amplicon end Chr3:789513
wt amplicon size 1272 bp
Locus specific primer 09fb: 5'-CGATCATAATCTCTTCGATGTTCA-3'
2nd locus specific primer 11sq: 5'-AAACACTGGACTTCACGAAGTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11