Genotyping and primer details


Genotyping and primer details for 110H12 - At2g41620
Line ID 110H12
Locus At2g41620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17352949 of line 110H12 Chr2:17352949
   
Insertion allele amplicon 8474 - SR39
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SR39: 5'-CAATCATATGGTCCTGAAAAGGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17352354
   
wt amplicon (over InsPos) 24sq - SR39
wt amplicon start Chr2:17352354
wt amplicon end Chr2:17353264
wt amplicon size 911 bp
Locus specific primer SR39: 5'-CAATCATATGGTCCTGAAAAGGTT-3'
2nd locus specific primer 24sq: 5'-GTATGCACTCTAGCTTTCATGCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11