Genotyping and primer details


Genotyping and primer details for 111B05 - At3g27980
Line ID 111B05
Locus At3g27980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10395026 of line 111B05 Chr3:10395026
   
Insertion allele amplicon 8474 - IH61
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IH61: 5'-GAACTTGTTGTGATGTTGCAGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10395536
   
Insertion allele amplicon 3144 - IH62
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IH62: 5'-ATTCCTTTTCCTTAGAGATCAGCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10394640
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11