Genotyping and primer details


Genotyping and primer details for 112B10 - At3g12320
Line ID 112B10
Locus At3g12320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3925011 of line 112B10 Chr3:3925011
   
Insertion allele amplicon 8474 - 07sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07sf: 5'-CTTTTTGTTTCTGGTTTGAAGGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3924636
   
wt amplicon (over InsPos) 24tf - 07sf
wt amplicon start Chr3:3924636
wt amplicon end Chr3:3925412
wt amplicon size 777 bp
Locus specific primer 07sf: 5'-CTTTTTGTTTCTGGTTTGAAGGAG-3'
2nd locus specific primer 24tf: 5'-TTAAAAAGGGGACCAGATTCTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11