Genotyping and primer details


Genotyping and primer details for 112G11 - At5g54530
Line ID 112G11
Locus At5g54530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:22153207 of line 112G11 Chr5:22153207
   
Insertion allele amplicon 8474 - 02ey
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02ey: 5'-TTCACTGTACCACTTCTATTCCCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:22153740
   
wt amplicon (over InsPos) 03fw - 02ey
wt amplicon start Chr5:22152683
wt amplicon end Chr5:22153740
wt amplicon size 1058 bp
Locus specific primer 02ey: 5'-TTCACTGTACCACTTCTATTCCCC-3'
2nd locus specific primer 03fw: 5'-CTTCTTTCCCATTTAAGATCCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11