Genotyping and primer details


Genotyping and primer details for 113E07 - At3g55260
Line ID 113E07
Locus At3g55260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20490307 of line 113E07 Chr3:20490307
   
Insertion allele amplicon 8474 - 06ja
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06ja: 5'-TTCTTGTGGTTTCAGGCTAATACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20489915
   
wt amplicon (over InsPos) 09ji - 06ja
wt amplicon start Chr3:20489915
wt amplicon end Chr3:20490801
wt amplicon size 887 bp
Locus specific primer 06ja: 5'-TTCTTGTGGTTTCAGGCTAATACA-3'
2nd locus specific primer 09ji: 5'-CTTCACAGTGGAAATCAAAAACAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11