Genotyping and primer details


Genotyping and primer details for 117C02 - At1g69380
Line ID 117C02
Locus At1g69380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26081860 of line 117C02 Chr1:26081860
   
Insertion allele amplicon 8474 - KM76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KM76: 5'-GTTGCAAAACTCTATGGAGAAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26081479
   
Insertion allele amplicon 3144 - KM77
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer KM77: 5'-TGATTCTCAAAGTTGGTTTGTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26082196
   
wt amplicon (over InsPos) KM77 - KM76
wt amplicon start Chr1:26081479
wt amplicon end Chr1:26082196
wt amplicon size 718 bp
Locus specific primer KM76: 5'-GTTGCAAAACTCTATGGAGAAAGG-3'
2nd locus specific primer KM77: 5'-TGATTCTCAAAGTTGGTTTGTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11