Genotyping and primer details


Genotyping and primer details for 117E05 - At1g30860
Line ID 117E05
Locus At1g30860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:10987363 of line 117E05 Chr1:10987363
   
Insertion allele amplicon 8474 - 04vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04vh: 5'-TCAGTGTCTTGAGAACTCCATGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:10987145
   
wt amplicon (over InsPos) 31vu - 04vh
wt amplicon start Chr1:10987145
wt amplicon end Chr1:10987997
wt amplicon size 853 bp
Locus specific primer 04vh: 5'-TCAGTGTCTTGAGAACTCCATGAT-3'
2nd locus specific primer 31vu: 5'-GCAAGATAGAGGAAGCAACGTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11