Genotyping and primer details


Genotyping and primer details for 117F08 - At1g04990
Line ID 117F08
Locus At1g04990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1421013 of line 117F08 Chr1:1421013
   
Insertion allele amplicon 8474 - 10ht
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10ht: 5'-TACCTCTGTTCTTCCTCGTCATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1421600
   
wt amplicon (over InsPos) 02io - 10ht
wt amplicon start Chr1:1420766
wt amplicon end Chr1:1421600
wt amplicon size 835 bp
Locus specific primer 10ht: 5'-TACCTCTGTTCTTCCTCGTCATTT-3'
2nd locus specific primer 02io: 5'-ATGTACTCATCCAAACAACAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11