Genotyping and primer details


Genotyping and primer details for 119D02 - At5g64710
Line ID 119D02
Locus At5g64710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25871750 of line 119D02 Chr5:25871750
   
Insertion allele amplicon 8474 - 09kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09kh: 5'-ACGGTTTTTACATAGAACCAATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25872202
   
wt amplicon (over InsPos) 13ky - 09kh
wt amplicon start Chr5:25871254
wt amplicon end Chr5:25872202
wt amplicon size 949 bp
Locus specific primer 09kh: 5'-ACGGTTTTTACATAGAACCAATCG-3'
2nd locus specific primer 13ky: 5'-CCAGAGAAACAATGGCTAAGAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11