Genotyping and primer details


Genotyping and primer details for 119F07 - At2g47850
Line ID 119F07
Locus At2g47850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19596417 of line 119F07 Chr2:19596417
   
Insertion allele amplicon 8474 - 08et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08et: 5'-CTGACTGTGCTCAATTTTGTGATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19596104
   
wt amplicon (over InsPos) 05fo - 08et
wt amplicon start Chr2:19596104
wt amplicon end Chr2:19596766
wt amplicon size 663 bp
Locus specific primer 08et: 5'-CTGACTGTGCTCAATTTTGTGATT-3'
2nd locus specific primer 05fo: 5'-AGTTTTCAAGTAAAACTGCCAGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11