Genotyping and primer details


Genotyping and primer details for 119F12 - At3g14070
Line ID 119F12
Locus At3g14070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4661444 of line 119F12 Chr3:4661444
   
Insertion allele amplicon 8474 - 05nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05nt: 5'-TCCAAGGAGATTAACAATTCCATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4661878
   
wt amplicon (over InsPos) 32oj - 05nt
wt amplicon start Chr3:4661131
wt amplicon end Chr3:4661878
wt amplicon size 748 bp
Locus specific primer 05nt: 5'-TCCAAGGAGATTAACAATTCCATC-3'
2nd locus specific primer 32oj: 5'-ATACATAGCTCTTTAAGCAGCCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11