Genotyping and primer details


Genotyping and primer details for 122E09 - At5g27610
Line ID 122E09
Locus At5g27610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9769675 of line 122E09 Chr5:9769675
   
Insertion allele amplicon 8474 - TM22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer TM22: 5'-TTGGATCATCATTCCTTAAACACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9769290
   
wt amplicon (over InsPos) 51rd - TM22
wt amplicon start Chr5:9769290
wt amplicon end Chr5:9770056
wt amplicon size 767 bp
Locus specific primer TM22: 5'-TTGGATCATCATTCCTTAAACACA-3'
2nd locus specific primer 51rd: 5'-CCATTTGGCATTGTTCTAGAATTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11