Genotyping and primer details


Genotyping and primer details for 122H12 - At3g17920
Line ID 122H12
Locus At3g17920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6141539 of line 122H12 Chr3:6141539
   
Insertion allele amplicon 8474 - 06uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06uj: 5'-ATCGAAGAATATTTTGGACACACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6142153
   
wt amplicon (over InsPos) 20vl - 06uj
wt amplicon start Chr3:6141277
wt amplicon end Chr3:6142153
wt amplicon size 877 bp
Locus specific primer 06uj: 5'-ATCGAAGAATATTTTGGACACACC-3'
2nd locus specific primer 20vl: 5'-TTCTTCTTCTGTTTGCTGATTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11