Genotyping and primer details


Genotyping and primer details for 124E07 - At1g18830
Line ID 124E07
Locus At1g18830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6490276 of line 124E07 Chr1:6490276
   
Insertion allele amplicon 8474 - 12sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12sm: 5'-CCAACAAATAGTTCGGTTGTCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6490757
   
wt amplicon (over InsPos) 02sx - 12sm
wt amplicon start Chr1:6489723
wt amplicon end Chr1:6490757
wt amplicon size 1035 bp
Locus specific primer 12sm: 5'-CCAACAAATAGTTCGGTTGTCTTT-3'
2nd locus specific primer 02sx: 5'-AGATTTGTCAAAACACAAAGGACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11