Genotyping and primer details


Genotyping and primer details for 125A01 - At1g31760
Line ID 125A01
Locus At1g31760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11373399 of line 125A01 Chr1:11373399
   
Insertion allele amplicon 3144 - 05fp
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 05fp: 5'-ACAATTCCTTCTCTAAAGCAACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11373111
   
wt amplicon (over InsPos) 10gf - 05fp
wt amplicon start Chr1:11373111
wt amplicon end Chr1:11373951
wt amplicon size 841 bp
Locus specific primer 05fp: 5'-ACAATTCCTTCTCTAAAGCAACCA-3'
2nd locus specific primer 10gf: 5'-GATATCGATTAGGGTTTCCACTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11