Genotyping and primer details


Genotyping and primer details for 125B11 - At2g47010
Line ID 125B11
Locus At2g47010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19318528 of line 125B11 Chr2:19318528
   
Insertion allele amplicon 8474 - 06vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06vh: 5'-CTCAAAAATGGAAACTACGACACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19318009
   
wt amplicon (over InsPos) 30vu - 06vh
wt amplicon start Chr2:19318009
wt amplicon end Chr2:19319215
wt amplicon size 1207 bp
Locus specific primer 06vh: 5'-CTCAAAAATGGAAACTACGACACA-3'
2nd locus specific primer 30vu: 5'-TCTGCAATTGCTTCATCTTCTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11