Genotyping and primer details


Genotyping and primer details for 125H07 - At4g33970
Line ID 125H07
Locus At4g33970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16280987 of line 125H07 Chr4:16280987
   
Insertion allele amplicon 8474 - 06dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 06dr: 5'-ATTTTCAGACCCATTTAACACCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16281595
   
wt amplicon (over InsPos) 08ep - 06dr
wt amplicon start Chr4:16280380
wt amplicon end Chr4:16281595
wt amplicon size 1216 bp
Locus specific primer 06dr: 5'-ATTTTCAGACCCATTTAACACCAC-3'
2nd locus specific primer 08ep: 5'-ATCATCTGGTTGAGGTGACTCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11