Genotyping and primer details


Genotyping and primer details for 129C05 - At3g48860
Line ID 129C05
Locus At3g48860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18118827 of line 129C05 Chr3:18118827
   
Insertion allele amplicon 8474 - 23wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23wp: 5'-AGGTACAAATTTTCTGCACAGCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18119505
   
Insertion allele amplicon 8474 - AP33
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP33: 5'-GTCAGAATTTCAAGACGCAACATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18119065
   
Insertion allele amplicon 8474 - DE37
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DE37: 5'-TTCCTAATCTAATGCCGCCTAGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18118231
   
wt amplicon (over InsPos) AP33 - DE37
wt amplicon start Chr3:18118231
wt amplicon end Chr3:18119065
wt amplicon size 835 bp
Locus specific primer DE37: 5'-TTCCTAATCTAATGCCGCCTAGTA-3'
2nd locus specific primer AP33: 5'-GTCAGAATTTCAAGACGCAACATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11