Genotyping and primer details


Genotyping and primer details for 129D02 - At1g54130
Line ID 129D02
Locus At1g54130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20211553 of line 129D02 Chr1:20211553
   
Insertion allele amplicon 8474 - DN44
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer DN44: 5'-CCAGCTACAACAACAGTCGAATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20212038
   
wt amplicon (over InsPos) 14dy - DN44
wt amplicon start Chr1:20211054
wt amplicon end Chr1:20212038
wt amplicon size 985 bp
Locus specific primer DN44: 5'-CCAGCTACAACAACAGTCGAATTA-3'
2nd locus specific primer 14dy: 5'-TAGGGAAAGTTCTTTGATTCTCCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11