Genotyping and primer details


Genotyping and primer details for 129E06 - At2g38330
Line ID 129E06
Locus At2g38330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16065799 of line 129E06 Chr2:16065799
   
Insertion allele amplicon 8474 - 10sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10sj: 5'-ACTTTGATTTGAGGAGAGAGCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16066096
   
wt amplicon (over InsPos) 11tc - 10sj
wt amplicon start Chr2:16065392
wt amplicon end Chr2:16066096
wt amplicon size 705 bp
Locus specific primer 10sj: 5'-ACTTTGATTTGAGGAGAGAGCAAA-3'
2nd locus specific primer 11tc: 5'-CTATCTCTCGGGTCTGATTTCTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11