Genotyping and primer details


Genotyping and primer details for 129H03 - At3g09070
Line ID 129H03
Locus At3g09070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2768930 of line 129H03 Chr3:2768930
   
Insertion allele amplicon 3144 - 07vo
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 07vo: 5'-CGGCGATAGGAAAGTGAATAGTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2769366
   
wt amplicon (over InsPos) 71wa - 07vo
wt amplicon start Chr3:2768217
wt amplicon end Chr3:2769366
wt amplicon size 1150 bp
Locus specific primer 07vo: 5'-CGGCGATAGGAAAGTGAATAGTAA-3'
2nd locus specific primer 71wa: 5'-GTGACCTAATAAGCTCGACGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11