Genotyping and primer details


Genotyping and primer details for 132F02 - At3g13460
Line ID 132F02
Locus At3g13460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4385612 of line 132F02 Chr3:4385612
   
Insertion allele amplicon 8474 - 03gz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03gz: 5'-CTGTCCCATCTTTCTGTTTTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4386086
   
wt amplicon (over InsPos) 11hv - 03gz
wt amplicon start Chr3:4385199
wt amplicon end Chr3:4386086
wt amplicon size 888 bp
Locus specific primer 03gz: 5'-CTGTCCCATCTTTCTGTTTTTCTC-3'
2nd locus specific primer 11hv: 5'-CGTTAAACGGGATTGTTCATATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11