Genotyping and primer details


Genotyping and primer details for 132G01 - At4g39240
Line ID 132G01
Locus At4g39240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18270509 of line 132G01 Chr4:18270509
   
Insertion allele amplicon 8474 - 08vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08vf: 5'-TTATTAACACCCAAACCAGACTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18270068
   
wt amplicon (over InsPos) 29vu - 08vf
wt amplicon start Chr4:18270068
wt amplicon end Chr4:18271226
wt amplicon size 1159 bp
Locus specific primer 08vf: 5'-TTATTAACACCCAAACCAGACTCC-3'
2nd locus specific primer 29vu: 5'-AAAGCAAAAACATAGCTGGAAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11